Sentence examples for ice cold condition from inspiring English sources

500 ul type I collagen gel solution (0.3%) containing 5 mg/ml of human fibronectin (Collaborative Research Inc. Lexingtonf, USA), 100 ul 10 × DMEM and 400 ul NaOH-Heppers buffer was mixed in an ice cold condition and pipetted into a 6-well plate and kept for 30 min at 37°C or gelatinization.

Au nanospheres were synthesized using the well-established citrate reduction method [14], where, 20 mL of 2.5 mM of HAuCl4 and tri sodium citrate were taken in a 50 mL round-bottom flask and stirred under ice cold conditions.

Briefly, sciatic nerve homogenate was mixed with 1 ml of trichloroacetic acid (4%) in ice cold conditions and centrifuged at 2000 r.p.m. for 10 minutes.

For determination of hydrogen peroxide (H2O2) content [ 29], 0.8 g roots were crushed with 8.0 mL of trichloroacetic acid (TCA) (0.1%, w/v) in ice cold conditions and the homogenate was centrifuged at 14,000 g for 20 min. 4 mL reaction mixture contained 1 mL supernatant, 1 mL PBS, and 2 mL potassium iodide (KI) and the absorbance was read at 390 nm.

The citrate-stabilized silver seeds were prepared using sodium borohydride as a reducing agent under ice-cold condition.

Graphite powder (2 g) was treated with concentrated sulphuric acid (46 ml) in ice-cold condition and stirred for 2 h.

Highly spherical Au nanospheres with an average diameter of 3.5 ± 0.5 nm (Additional file 1) were observed, depicting the controlled citrate reduction method performed at the ice-cold condition.

Typically, 20 mL aqueous solution containing 2.5 × 10−4 M AgNO3 and 2.5 × 10−4 M tri-sodium citrate was taken in a two-necked round bottom flask and stirred under ice-cold condition.

10%% skin homogenate was prepared in 50 mM Tris HCl (pH 7.4) under ice-cold condition.

All the steps were performed in ice-cold conditions.

Both root and leaf tissues (>200 mg) from water hyacinth seedlings were homogenized separately in a pre-chilled mortar and pestle under ice-cold conditions with 2.0 ml of extraction buffer [50 mM phosphate buffer (pH 7.5), 0.5 mM ascorbate and 1 mM EDTA].