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For detection of the pancreatic insulin content, whole pancreases were homogenised in ice-cold acidic ethanol (0.1 mol/l HCl in 70% ethanol) and incubated for 24 h at 4°C.
To each tube, 5 mL ice cold 5% acidic phenol (Sigma Aldrich)/95% ethanol (Kemetyl Norge, Vestby, Norway) were added and the mixture was kept on ice for 20 min to stabilize the mRNA [ 7].
Subsequently, each well was gently treated with 1 mL of an ice-cold mildly acidic medium (sodium acetate buffer, pH 4.5) to remove surface-bound radioactivity, and the rinses were collected in tubes (acidic wash).
"Her hands were ice cold.
Warm or ice cold?
He was ice cold.
It was ice cold in the car.
The market was ice cold.
This man is ice cold.
Ice cold to the last.
His feet are ice cold.
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