Exact experimental procedures are as follows: 97 mL of distilled water was placed in a 250 mL glass beaker in an ice bath.
Aqueous solutions (50 mL) of GG (0.5% (w/v)), prepared in glass beaker, were kept, during sonication, in an ice bath which allowed to keep an average temperature of the samples below 25°C.
TCE was added to soil in a glass beaker that was immediately covered and placed on ice to avoid TCE volatilization.
Brain tissues (10 mg; wet weight) were homogenized in BN-sample buffer 1 (250 mM Sucrose, 30 mM morpholine-propane sulfonate buffer, 0.2 mM phenylmethylsulfonyl fluoride, pH 7.2) using a homogenizer with a tight-fitting Teflon pestle (1 min at 500 rpm) and kept cold by immersing the homogenizer in an ice-filled beaker.
Glass beakers and measuring cups.
In the morning, saliva was stimulated by chewing Parafilm® and collected into ice-cooled beakers.
After stimulation by chewing Parafilm®, human whole saliva from 20 healthy volunteers of both sexes was collected into ice-cooled beakers, pooled, centrifuged, dialyzed, and lyophilized for storage.
In order to form a salivary conditioning film, human whole saliva from at least 20 healthy volunteers of both genders was collected into ice-cooled beakers after stimulation by chewing Parafilm®.
Around 25 numbers having around 30 g of total weight of well-grown matured worms (E. foetida) of approximately equal length were applied cold stress by keeping them in chilled conditions, in ice cubes in beaker at −5 °C for 3 4 min.
Place 150 ml sucrose saline, and empty 50 ml beaker on ice.
Cut these with fine scissors and remove brain to 50 ml beaker on ice.
