Sentence examples for ice adherent from inspiring English sources

Cell medium containing detached cells was transferred to tubes and placed on ice, adherent cells were washed with PBS, trypsinized and added to the proper medium samples.

Tissues were placed into ice cold PBS and freed of adherent fat.

Surface labelling was performed with FITC-labelled antibodies to CD14 (DAKO, Denmark, 1 100 dilution) for 45 min directly on adherent viable PBMLs under ice bath.

Half of these BALC** were adhered to plastic for 2 hours, rinsed three times with PBS to remove any non-adherent cells, and adherent cells detached with ice-cold PBS-EDTA (BALC***).

Adherent cells were put on ice.

Adherent cells were harvested on ice, and infected with late stationary phase L. donovani promastigotes.

Briefly, plates of adherent cells were washed twice in ice-cold PBS then twice in ice-cold cytoskeletal buffer (CSK: 10 mM PIPES/KOH pH 6.8, 100 mM NaCl, 300 mM Sucrose, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 1 cOmplete™ Protease Inhibitor Cocktail per 50 ml) and drained at a shallow angle on ice for 2 min.

Plates were rinsed twice with PBS, and adherent cells were scraped in ice-cold PBS and collected by centrifugation.

The tumours at each time point were pooled together, macroscopically inspected, the adherent tissues removed, rinsed in ice-cold PBS, and divided.

The rats were then sacrificed by cervical dislocation, and the heart was immediately removed, placed in ice-cold Krebs' buffer, carefully cleaned of adherent fat and connective tissue, weighted and divided in left and right ventricle.

Synovial tissue was taken on ice and prepared within 2 h by removing adherent non-synovial tissue.