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We acquired an iAs standard of 1,000 mg/L from the National Center for Standard Reference Materials (Beijing, China).
The standard reference materials used were iAs standard of 1000 mg/L from the National Center for Standard Reference Materials (Beijing, China) and a mixed arsenic standard of 1000 mg/L MMA and DMA (Tri Chemical Laboratories Inc., Yamanashi, Japan).
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To permit statistical evaluation of these aggregation indices, a reference curve holding mean IAs and standard deviations for 1,000 randomly generated datasets containing 5 200 genes was computed.
Oligonucleotides were synthesized at Integrated DNA Technologies Coralvillee, IA) using standard phosphoramidite chemistry.
Reported cpm was adjusted for variation in nonspecific binding by subtraction of the cpm of the IA-2βA negative IA-2βA negative
Oligonucleotides were supplied by IDT (Coralville, IA) as standard, desalted oligos and were used without further purification.
All reagents were molecular biology grade or better from Sigma (Sigma-Aldrich, St . Louis MO) and oligonucleotides were synthesized by IDT (Coralville, IA) with standard purification.
Oligonucleotide primers were synthesized and purified by Integrated DNA Technologies IDTT; Iowa City, IA, USA) using standard desalting.
In the 1980s astronomers began to use Type Ia supernovae as standard candles.
The importance of Type Ia SNe as standard candles for cosmology makes the study of their progenitor systems particularly important.
The 1998 announcements that the universe's expansion is speeding up (Science, 30 January 1998, p. 651) relied on this technique, using exploding stars dubbed type IA supernovae as standard candles.
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