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To further elucidate the region containing the iAs responsive element, we examined a series of deletions of the 5′-flanking region of K16 gene.
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To confirm that the K16 ARE indeed functions as an iAs-responsive transcriptional control element, we performed transient transfections of HaCaT cells with a p3xARE/Luc construct and then subjected these cells to iAs for 6 hr.
Our current results are consistent with these earlier studies in showing that the formation of the iAs-responsive NRF2/ARE complexes is reduced by a mutation in the GC box.
In addition, recent epidemiological studies confirm that iAs significantly increases DNA hypermethylation in a dose-responsive manner at the promoters of oncogenes [ 56, 57].
Such analyses will likely identify the genes responsive to the long-term exposure to low dose of iAs (and iAs-transformation) on a genome-wide scale as well as their functional roles.
The responsive cell lines all harboured H1047R mutations in PIK3CA and have high levels of p110α and class-Ia PI3K activity.
Servers are responsive.
"He was very responsive.
Politicians are actually pretty responsive.
They were very responsive".
He was responsive.
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