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As a practical matter, I was activated by two things.
After removal of excess cIgE, surface FcεRI was activated by crosslinking the receptor-bound ligand with haptenized antigen.
At the time of activation, the pro-TPPI was incubated with 25 µM NBS, 10 mM CaCl2 or 10 mM EGTA for 15 min. Following this preincubation, the pro-TPPI was activated by autocatalysis in 50 mM ammonium formate buffer, pH 3.5 for 5 min at 37°C, and finally TPPI activity was measured by adding the substrate GFFL-AFC.
SMP were diluted to 5 mg/ml in SET buffer pH 7.5 and complex I was activated in the presence of an NADH-regenerating system.
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I was activating a subjective circuit of joy and sadness.
I created a new Defiant (one of our player factions) at launch, and within my first hour of play I was activating a huge construct to cut a path through a horde of monsters blocking my way.
Within this complex, TβRI is activated by TβRII through phosphorylation, and the activated TβRI phosphorylates receptor-regulated Smad2 and Smad3 (R-Smads) at two serine residues in the C-terminus [ 2, 5].
I'm activated.
By photoaffinity labeling human atrial membranes with [ 32 P]azidoanilido-GTP, followed by immunoprecipitation with antibodies specific for Galpha(i), we found that Galpha(i) is activated by stimulation of beta(2)ARs but not of beta(1)ARs.
From Theorem 1, we know that every link e i = (u i, v i ) after time t i is activated at each of its reserved TU and that u i has a queue of length q(e i, t i ) at the beginning of each TS interval.
We asked whether I-SceI-induced HR is influenced by the cell cycle stage during which I-SceI is activated.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com