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Meiotic prophase I staging and the degree of chromosome synapsis in Bscl2+/+ and Bscl2−/− spermatocytes were determined by co-immunochemical detection of the SYCP1 and SYCP3 using polyclonal mouse anti-SYCP3 (Abcam, Cambridge, MA, USA) and polyclonal rabbit anti-SYCP1 (Abcam) antibodies at a 1 : 500 dilution in dilution buffer (1 mg/ml BSA (Sigma) in 1 × PBS, 0.01% Triton X-100).
CKD was defined according to the K/DOQI staging [ 13].
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Could I stage a marathon comeback?
So do I stage a personal boycott?
Only the oocytes remaining at the metaphase I stage were used for in-vitro maturation.
The different sections of 527 Fault had different activity intensities during the main rift I stage.
During the main rift I stage, the Qingxi Sag underwent its most active tectonic subsidence.
Then I stage that house.
"Shh - you hear that?" I stage-whisper.
Of all the scenes, I staged one.
Phase I stage.
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