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The cell pellet was resuspended in 400 µl of Cytoplasmic Extraction Reagent I solution containing protease inhibitors.
A promising approach for conductive patterns with high efficiency for flexible electronics was developed by direct-writing, silver(I) solution (silver nitrate, acetate silver, etc).
Two microlitre of DNase I solution (concentration series) or serum samples were added to the wells.
Slowly the supernatant was removed and to the pellet added 100 μl fresh ice-cold Otto I solution.
Briefly, tracheas were digested with dispase I solution (2.5 U/ml dispase I, Roche) for 2 h at 37°C.
In brief, small pieces of tissues were fixed in ice cold (2°C) HOPE I solution for 1 d to 3 d.
BrdU (10 µM) was added to cell cultures during the final 60 min. Cells were harvested and fixed and resuspended in DNase I solution as described [47].
The dermal tissue was minced and digested thoroughly with 30 volumes of 200 U/ml collagenase I solution (Sigma, USA) (200 U collagenase I solution in 1 ml PBS) at 37°C for 2 hours, followed by cell collection by centrifugation.
A category I solution to ADPMAN was proposed by SMILEY.
Collagen I solution was prepared as already described [ 8].
Using ED as isolation method, 0.2% collagenase I solution was used.
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