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TSEs also exhibit differences in PrPSc glycosylation patterns; Ricinus communis agglutinin I (RCA I) binding to PrPSc varies between vCJD- and sCJD-derived PrPSc [16] and ELISAs with RCA I and Datura stramonium lectin (DSL) can be used to discriminate between normal and scrapie affected tissues [17].
Of the positively reacting lectins, the ones producing the most specific signal were Datura stramonium agglutinin (DSL), which recognizes (β→1,4) linked N-acetylglucosamine oligomers and Ricinus communis agglutinin I (RCA I), which recognizes oligosaccharides ending in galactose and N-acetylgalactosamine (Fig S1 and data not shown).
Besides WGA, another lectin Ricinus communis agglutinin I (RCA I) has been used for O-GlcNAc enrichment as well.
Ulex europaeus agglutinin I (UEA), Ricinus communis agglutinin I (RCA), peanut (Arachis hypogaea) agglutinin (PNA), and Maackia amurensis lectin II (MALII) were from Vector Laboratories (Peterborough, UK).
After washing, slices were incubated for 30 min in blocking solution (0.3% Triton + 10% NGS in PBS) and then with Biotinylated Ricinus Communis Agglutinin I (RCA I, B-1085 Vector Laboratories; 1 : 200) in blocking solution for 30 min. Slices were then washed in PBS and incubated in Tris HCl 100 m m (5 min), and staining was revealed using a biotinylated secondary antibody.
The binding specificity of lectins Ricinus communis agglutinin I (RCA), peanut (Arachis hypogaea) agglutinin (PNA), Maackia amurensis lectin II (MALII), and Ulex europaeus agglutinin I (UEA) was utilized in force spectroscopy measurements to quantify the affinity and spatial distribution of their cognate sugars at the molecular scale.
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Patient-derived tissue microarrays were then employed to analyze whether the staining of Ricinus communis agglutinin I (RCA-I), correlated with TNBC severity.
Surprisingly, we found that a single terminal galactose-specific lectin, Ricinus communis agglutinin I (RCA-I), binds to these cells to a degree that is proportional to their metastatic capacity.
Meanwhile, Heather Headley, who burned up the stage in "The Lion King" and "Aida," has just released "This Is Who I Am" (RCA), a slickly produced R & B album that appears to be her farewell to Broadway.
THE STROKES' "Is This It" (RCA).
In 2001, the band released "Is This It" (RCA), a debut album that helped popularize a musical movement that's now easy to take for granted.
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