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The first two shots are from an English magazine and the last shot is from one of many attempts I performed using a Spanish language song.
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The song that I performed using origami on my tour is new.
MALDI-IMS/MSI were performed using a Q-Star Pulsar i hybrid quadrupole time-of-flight mass spectrometer (Applied Biosystems/MDS Sciex, Concorde, Ontario, Canada) fitted with a variable repetition rate Nd YV04 laser set at 5 kHz.
The long-term confocal imaging of aggregates in cells treated with cucurbitacin I was performed using a Pathology Devices stage incubator (maintaining 37°C and 5% CO2) on a Nikon A1R confocal microscope.
Collagen I quantification was performed using a Macro in ImageJ to identify and quantify collagen I positive areas.
SYBR Green I qPCR was performed using a 7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA).
A phase I study was performed using a single oral dose every 3 weeks, administered as a suspension reconstituted in 0.1% Tween 80 and 0.9% saline.
Annexin-V/PI assays were performed using a commercial apoptosis assay kit (Roche, Switzerland) according to the manufacturer's instructions.
The staining of PCR products with SYBR Green I (Lonza) was performed using a shallow well MMV (0.1 μL/well) according to the manufacturer's instructions.
Four hours after the intravenous injection of 220 MBq I-mIBG, cardiac I-mIBG scintigraphy was performed using a double-headed gamma camera (Siemens Symbia E, Siemens Healthcare, Erlangen, Germany) equipped with low-energy, high-resolution, parallel-hole collimators.
Immunohistochemical staining for type I collagen (Col-I) was performed using an affinity-purified goat anti-mouse Col-I antibody (Southern Biotechnology Associates) as described previously.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com