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And that's how I mutated from Gandhi Girl to Army Wife.
I mutated the short story into some joyless academic exercise, which provided an excuse for not writing any.
Besides, a major influence of the PrPc expression levels has been demonstrated in transgenic mice over-expressing (i) mutated human prion protein linked to Gerstmann Sträussler-Scheinker syndrome [37], [38] or (ii) a prion protein with a nine-octapeptide insertion associated with a human familial prion disease [39], [40].
A database search revealed 14 potential serine/threonine and two tyrosine phosphorylation sites, which I mutated to alanine and phenylalanine, respectively.
As half of sgRNAs have been reported to fail in C. elegans (Kim et al. 2014), I mutated two PAMs in the oligo to allow knock-in attempts with different sgRNAs.
During my initial design of the nhr-23 :: 2×FLAG repair oligo, I mutated two potential PAMs, as it has been reported that half of all sgRNAs fail; this single oligo would allow testing of two separate sgRNAs (Kim et al. 2014).
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In order to avoid the UFA production caused by fadR overexpression, FadR regulon was rewired by i) mutating FadR consensus binding sites of fabA or fabB, ii) integrating fabZ into fabI operon, and iii) enhancing the strength of fabI promoter.
Swarm individuals F i mutating on nearby inputs (measured with the Hamming metric) are assigned to the same cluster, whereas distant populations share different neighborhoods.
As they mutate, I mutate with them, like a sentence continually revised.
The probability of observing a particular nucleotide i mutating into j (i ≠ j) during interval τ is p ij ≃ g ijτ, where g ij ≥0) is the rate of change i → j per time unit.
The infinitesimal rate matrix Q : = (q i, j ) i, j = i min, …, i max is a square matrix wherein each element q i, j specifies the relative instantaneous rate of allele i mutating to allele j, and the shared lower and upper bounds of i and j are imin and imax, respectively.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com