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Taken together, we can postulate that the wild-type pol ι may require a low level of Mn2+ rather than Mg2+ as a metal for performing proper polymerase function for two reasons: (i) mediating efficient catalysis and (ii) facilitating tight DNA substrate binding, although the structural basis for such a scenario has not been revealed yet.
In a previous study, we showed that there is a prominent role for FcγRI mediating chondrocyte death during ICA.
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TH gene expression induced by TCDD exposure would be regulated by AHRE-III mediated mechanism independent of the conventional AhR-mediated pathway.
I mediated the chaos of potential loss by embracing it.
Now I mediate about 20 minutes in the morning and 20 minutes in the evening.
"I mediated between the two sides, and both sides used me to reach common ground until March 15," he said.
Two modules in Pol I mediate the narrowing of the DNA-binding cleft by closing the clamp domain.
In this study, we further improved homologous integration frequencies by combining I-SceI mediated double strand break with disruption of the tku70 gene.
The inability of the tku70 mutant to repair a I-SceI mediated DSB via NHEJ was used to force integration of an expression cassette with homologous flanks surrounding the DSB site.
Open image in new window Fig. 1 Activity of acylated triterpene saponins 1-10 on topoisomerase I mediated relaxation (R) of supercoiled pBR322 DNA (S) in a dose dependent manner.
To test whether DNA-PLA-PEG-NPs can protect the loaded plasmid DNA from nucleases digestion, the results of DNase I mediated digestion was evaluated using agarose gel electrophoresis [23].
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