Exact(11)
(i) Extraction solutions: The extractants tested were hexane (5 mL) or a solvent/MeOH mixture (always 2 + 1 v/v, 5 mL) where the solvents were DCM, chloroform, toluene, acetone, Et2O, MTBE, EtOAc, or AcN.
The extracts were accessed in terms of: (i) extraction yield (EY), (ii) total phenolic content by Folin Ciocalteu method (TPC), (iii) phenolics quantified by high performance liquid chromatography (PC-HPLC), and (iv) antioxidant activity (AA).
Furthermore, the study evaluated only class I extraction and non-extraction patients with a mean FMA of 28.37° (SD 3.96) and 26.85° (SD 5.86) respectively.
The product system was defined by the following steps: (i) extraction and manufacturing of raw materials (upstream flows), (ii) external energy supplied to the system, (iii) nuclear power plant, and (iv) hydrogen production plant.
A large amount of literature deals with this problem, by applying a variety of different techniques, which we briefly review in Section 2; most approaches however share a common high-level structure: (i) extraction of features from the video data, (ii) if necessary, dimensionality reduction of feature vectors, by means of techniques such as PCA, (iii) classification of the sequence. .
The process includes three major steps: i) extraction of all influenza A protein sequence data, processing and definition of data sets comprising relevant human and avian virus groups; ii) entropy analysis of sequence variability and identification of conserved peptide sequences of 9 amino acids or longer; and iii) prediction of supertype-restricted, HLA-binding sequences.
Similar(49)
DNA was subsequently extracted using the automated MagNA Pure LC with the DNA-I extraction kit on the high performance protocol (Roche Diagnostics, Auckland, New Zealand).
Nidhi R and AJ did DNA extraction, pooling, PCRs, and Cel-I extraction.
RNA-linker mix was purified by phenol-chloroform-isoamyl alcohol (P-C-I) extraction and ethanol/sodium acetate precipitation method and was dissolved in 14 μl of RNase-free H2O.
Buffer and enzyme were removed by P-C-I extraction again and the ligated RNA-linker was dissolved in 10 μl of RNase-free H2O. cDNA was synthesized by SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA) using random hexamers following the instructions of the kit.
Similar to that in the i-vector extraction, the i-supervector is computed as the posterior mean of a latent variable, but with a much higher dimensionality.
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