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DNase I treatment crucially prevented the amplification of intronless genes such as the Kir 2.0 family from contaminating genomic DNA.
The unique conformation of HLA-G as a homodimer of conventional, β2m-associated class I complexes crucially maintains recognition by the LILRB1 receptor because co-crystal structures confirm that a significant proportion of this receptor's interface with HLA-I molecules is with the β2m molecule 15.
Collectively, these results suggest that FACT has a specific function in rDNA transcription by Pol I and, crucially, that FACT appears to function at a stage following pre-initiation complex formation, initiation of transcription and transcription of the first 40 nt.
Even more crucially, I know I'll be so overwhelmed with the amazing birds I see that I'll be able to forget my exhaustion – or at least keep birding right through it.
Most crucially, I did not know that I'd been traveling on Thursday night with a companion of sorts: a second experiment participant at a remote computer who was playing a text-based game on an Internet browser that occasionally intersected with my live "Sleep No More" experience.
Crucially, I was also drunk.
But crucially, I was in the book group.
Crucially, I find I can put lipstick over the top and the colour still adheres.
Crucially, I feel the job offers me an opportunity to reboot myself professionally.
And, crucially, I assume you exclude everything that is not a table.
Crucially, I noticed how digital journalists assumed that everyone in the population was like them.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com