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Group I animals received estradiol injections; group II received estradiol and progesterone; and group III received estradiol, progesterone, and prolactin.
The Animals were randomly divided into 4 groups of 6 rats in each as follows: Group (I): Animals received rat chow only and considered as control.
Group I, animals received vehicle (10% DMSO in water, 10 ml/kg body weight), animals of group II received paracetamol 100 mg/kg body weight while animals of group III; group IV and group V were treated with 100; 200 and 400 mg/kg body weight of polyphenol-rich fractions dissolved in 10% DMSO 1 h orally before acetic acid injection.
In addition to baseline measurements, the following experimental protocol were used: (i) animals received the angiotensin converting enzyme (ACE) inhibitor enalapril in their drinking water (10 mg/kg/d), and (ii) to induce sepsis, mice received a single i.p. injection of lipopolysaccharide (LPS, Sigma-Aldrich, Munich, Germany, 3 mg/kg).
Group I animals received distilled water, Group II received Diclofenac sodium at 10 mg/kg while animals of Group III and Group IV were treated with 250 and 500 mg/kg of the ethanol extract of C. zedoaria after an overnight fast.
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However, it was observed that there is a trend of decrease of complexes I III activities in animals receiving carnosine, as compared to control rats).
We observed a significant decrease of complexes I III and II activities in animals receiving carnosine acutely, as compared to control group.
In the present study, we observed a statistically significant decrease of complexes I III and II activities in animals receiving carnosine acutely, compared to control group rats.
Following an 8-day rest phase II was run exactly as phase I except that all animals received an intravenous infusion of 30 mg·kg−1 CAT-354 in a volume of 3.2 mL·kg−1 over 20 min on days 1, 5 and 9 (70 min prior to antigen challenge).
Another group simultaneously received intravenous unfractionated heparin (ATIII + Hep, n = 5, sodium heparin, 100 IE/24 hours, i.v ., whereas additional animals received low molecular weight heparin (ATIII + LMWH, n = 5, fraxiparin, 5 μl/kg, 2 hours before LPS, s.c).. Saline-treated animals receiving only LPS.
(c) LPS plus HA-sham group (N = 6), animals received an i.v. infusion of saline with LPS.
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