It was a steamy night in May 2003 and I was coated with Bronx sweat and fumes from taking the boys up and down until the seventh inning stretch.
Within seconds of the band's opening notes, I was coated in a film of sugar that left me and my glasses residually sticky for days after the festival (I just hucked all my clothes).
Transwell migration assays were performed using the same procedure as that used in performance of the invasion assay, except that underside filters with type I collagen was coated.
Type-I collagen was coated onto alumina and bombarded with 50 keV He+ ions at doses between 2×1012 and 1×1015 ions/cm2.
To detect anti-AT-III IgG, maxisorp plates were coated with 10 μg/ml human AT-III in PBS.
S. mutans, SAI/II antigen or BSA were coated on ELISA plates (Nunc) at a concentration of 1 10 μg per well in PBS overnight at 4°C.
Briefly, 96-well plates (Immulon I, Dynatech, Chantilly, VA) were coated with recombinant PsaA (2 μg/ml, 100 μl per well) overnight at 4°C.
The undersides of membranes in CIM plates were coated with 10 μg/ml of either fibronectin or collagen I. Plates were coated with collagen I for 3 hours at room temperature, blocked in 5% BSA for one hour, and washed three times with PBS.
I was always coated in a scrim of sweat and ice cream and muffin batter and was often too drained at the end of the day to bathe.
"I am not sugar coating the challenges ahead but with determination, resolve, and sheer political will, this city can and will come through stronger the other side.
