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We observed that the inner region of spheroid was hypoxic (data not shown).
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Similarly to Chandrasekaran and Price [ 49], we find that a value for κ of 1.0 strikes a balance between hard and soft bounds that yields reasonable predictions for our analysis of both hypoxic transition data and transcription factor knockout data.
Western blot analysis revealed that D-12 cells showed significant expression of VEGF and IL-8 in vitro and that the expression of both angiogenesis factors was up-regulated under hypoxic conditions (data not shown), consistent with the ELISA data.
As expected hypoxia-induced secretion of VEGF by MoDC was increased by incubation for 24 hours under hypoxic conditions (data not shown).
The number of arterioles quantified in LV myocardium was similar in sFlt-1 and vehicle-treated hypoxic embryos (data not shown), suggesting that the effect of sFlt-1 was independent from the cardiac vasculature.
For the hypoxic transition data set, we calculate a value of 0.48 (p = 1.7 × 10−5).
The dosR knockout and wild type hypoxic transition data are available at GEO accession GSE8829.
Addition of glucose did not modify the apoptotic levels in hypoxic cells (data not shown).
In no case we observed a significant downmodulation of melanoma TAA in cells cultured in hypoxic conditions (data not shown).
In CHEK-1 cells, the expression of CA9 protein did not increase under either normoxic or hypoxic conditions (data not shown).
The systemic blood pressure (BP) was unchanged between groups although the BP was lower in the 28-day hypoxic group (data not shown).
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