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Among the differentially expressed transcripts, considerably higher numbers of ESTs were represented as either hypothetical (unknown) proteins or no GenBank match suggesting those may be novel genes associated with internal defense of abalone.
Within this latter set, 131 (6.9%) are described as hypothetical, unknown or expressed proteins thus not confirming an effective functional role of the transcript product.
However, if the BLAST hit is to a sequence of uncertain function – indicated by descriptions like "hypothetical", "unknown" and "uncharacterized", a meaningful hit was selected.
In this study, we used the SEM which expresses the error of the hypothetical (unknown) "true score" based on the observed raw scores [ 36].
The sTOL is a fully resolved binary tree, with each internal node either being mapped onto a known ancestral species or left unlabelled as a hypothetical unknown ancestor.
Hypothetical unknown proteins (including the MbnC replacement in Group V operons) are present in several operons, and a range of proteins of unknown relevance, including several varieties of known copper-related proteins, appear in a few operons only.
Similar(53)
Down-regulated proteins are indicated by, whereas up-regulated proteins are indicated by, hypothetical or unknown proteins are indicated by ???
PSORTb, CDD, Motifscan and Pfam revealed function and subcellular localization for 27 of the 31 hypothetical and unknown proteins.
The other categories are as follows: secondary metabolism (4.64%), sulfur metabolism (3.31%), signal transduction (2.65%), lipid metabolism (1.99%), methylation (1.32%), cytoskeleton (0.66%), and hypothetical or unknown (4.64%).
While 52.34% of the evaluated protein spots were common across three species, the remaining 47.66% fraction mainly comprised of the hypothetical and unknown proteins.
Dark matter and dark energy both are hypothetical, mostly unknown, but thought to be vital forces in the universe.
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