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The hypothetical assignment was developed through discussion with three experienced public health physicians.
Frameworks for scoring the documents were developed: the planning documents, the hypothetical assignment and the additional question list.
The scores (1 5) for the hypothetical assignment and additional questions were also compared by means of the Mann-Whitney test, while the scores for reports were recoded and reported as 'used' or 'not used' research.
The weighted Kappa scores for interrater agreement on use of research information for reports, hypothetical assignment and additional questions were 0.50, 0.91 and 0.87 at pre-test respectively and 0.89, 0.75 and 0.74 at post-test.
Behaviour was considered the primary outcome and was measured by analysing the contents of local health service reports and of a hypothetical assignment, by a postal survey, a telephone survey and a questionnaire.
Similar(55)
Although the use of CATs as a learning assignment had not previously been reported in this context, hypothetical assignments have been used [ 16].
The latter observation implies that the hypothetical incorrect assignment of recent paralogy could not only involve the motif-free ribosomal genes but extend also to their flanking genes.
It is particularly useful when the genome of interest suffers from poor annotation due to low or no sequence similarity to known proteins, a significant problem for P. falciparum, as over 60% of the predicted open reading frames (ORFs) were annotated as "hypothetical" without functional assignment [ 5].
This methodology can assist in the assignment of hypothetical proteins to a known function, used by us, for example, in the case of the TonB-dependent receptors (Table 1).
The annotations can also be easily selected and retrieved according to species name, level of detail of the enzyme function prediction (four-field or three-field EC numbers), consistency or inconsistency between EFICAz and KEGG assignments, presence of the keywords "hypothetical" or "unknown" in KEGG assignments as of 2005, EC number and gene name.
Functional assignments for hypothetical and predicted genes of interest were performed manually on a gene-by-gene basis.
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