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The cell surface hydrophobicity of bacterial strains was assessed in the Bacterial Adhesion to Hydrocarbons (BATH) test (Rosenberg 1984).
Two complementary methods, namely, bacterial adherence to hydrocarbons (BATH) and salt aggregation test (SAT), were performed to assess bacterial surface hydrophobicity.
Surface hydrophobicity was assessed using the bacterial adherence to hydrocarbons (BATH) assay, with xylene (BDH, VWR International, Leicestershire, UK) as the hydrocarbon of choice [ 11].
Furthermore, bacterial adhesion to hydrocarbons (BATH) test was applied to measure the cell surface hydrophobicity, a property related to the structure and composition of cell surface [ 77].
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Cell surface hydrophobicity of biofilm-harvested cells and planktonic cells was measured by bacterial adhesion to hydrocarbon (BATH) assay [14].
The predominant n-hexadecane uptake mechanism was identified to be biosurfactant-mediated using bacterial adhesion to hydrocarbon (BATH) test and surface tension measurements.
Biosurfactant activity was evaluated by hemolysis, drop-collapsing test, lipase production, oil-spreading technique, bacterial adhesion to hydrocarbon (BATH) assay, penetration assay, and emulsification assay.
Biosurfactant assay was determined for the pure culture of actinobacteria by seven different methods, namely, (i) hemolysis, (ii) drop-collapsing test, (iii) lipase test, (iv) oil-spreading technique, (v) bacterial adhesion to hydrocarbon (BATH) assay, (vi) penetration assay, and (vii) emulsification assay.
Such uptake is facilitated by cultures that possess or can induce high cell surface hydrophobicity and cultures that show enhanced adherence to n-hexadecane in bacterial adhesion to hydrocarbon (BATH) assay.
Transmission electron microscopy revealed differences in cell surface architecture of MsmRv0024 when compared with Msm wild-type (WT) and vector control Msm pSMT3 (pstrainstrands and this aggregation pattern was due to increased cell wall hydrophobicity, as determined by Bacterial adhesion to hydrocarbons assay (BATH).
The endothermic (heat-absorbing) decomposition of the hydrocarbon near the molten bath cools the tuyeres and protects the adjacent refractory.
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