Afterward the slides were deparaffinized, hydrated, placed in 10 mM citrate buffer (pH 6) and microwaved for a total of 20 minutes for antigen retrieval.
In brief, the sections were deparaffinized, hydrated, and placed in peroxidase block solution for 10 minute.
Briefly, sections were deparaffinized, hydrated and placed in a peroxidase block solution for 10 minutes.
They were then deparaffinised, hydrated, and placed in prewarmed citrate buffer and boiled in a microwave oven for 20 min.
For immunostaining, slides were brought to room temperature, hydrated and placed in citrate buffer pH 6.0 (TA135-HBH; Thermo-Fisher, WalthaMA MA, USA).
The sample was hydrated by placing 2 μL of a 40% (v/v) glycerol/water solution in a sealed IR cell without touching the sample.
Each slide, after being deparaffinised and hydrated, was placed in a container and covered with 0.01 M sodium citrate buffer (pH 6.0) and heated in a microwave oven (500 W) four times for 5 min each.
Briefly, paraffin sections of 4 μm thickness were deparaffinised and hydrated, then placed in 0.2 M HCl for 10 min followed by digestion in 25 μg ml−1 proteinase K (Merck, Darmstadt, Germany) for 10 min at 37°C.
Three new leaves produced during the 10-week experiment in the growth chamber were harvested from each genotype and fixed in 70 % ethanol for 7 days and then hydrated by placing them in 70, 50, 25 and 0%% ethanol sequentially, for at least 15 min in each solution.
Here are some creative ways cricket keepers keep their quarry nice and hydrated: Try placing an inverted bottle reptile water dispenser with a sponge in the reservoir into your container.
