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Unidirectional interspecific hybridization linked to unilateral incompatibility is frequently described in plants.
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Prior to hybridization, oligonucleotides were cross linked to the slides by UV irradiation at 100 mj and the excess was removed from the arrays by washing them twice in one minute in 0.2% sodium dodecyl sulphate (SDS).
DNA was cross linked to the membrane, and PNA probes were hybridized to the membrane using conditions consistent with probe hybridization to nuclei on slides.
Linear models were used to calculate the significance of the variation in DNA hybridization intensities linked to the isolation sites and the identified genetic groups.
Polyploidy and hybridization are usually linked because interspecific hybrids are often sterile, and fertility may be restored by chromosome doubling.
All of the other candidates explored by in situ hybridization have been linked to annotated genes.
For each probe the log2 hybridization ratio was linked to genome annotation coming from the JGI website.
For each probe the hybridization ratio was linked to genome annotation coming from the DOE JGI website for a respective genome.
For each probe, the log2 hybridization ratio was linked to genome annotation from the Joint Genome Institute (JGI) website (http://genome.jgi-psf.org/Trire2/Trire2.home.html).html
Hybridization has been linked to activation of transposable elements in several organismal groups, with most examples documenting such activity in early generation hybrid genotypes (Waugh O'Neill et al. 1998; Labrador et al. 1999; Shan et al. 2005; Petit et al. 2010).
As with other asexual plant complexes, apomixis and hybridization are closely linked in H. perforatum [ 28], and interestingly the dosage of genetic factors has been proposed to influence the penetrance of apomixis, as tetraploid and hexaploid genotypes tend to be more apomictic and sexual respectively, regardless of geographic origin [ 29].
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