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The PNA-DNA hybrid probes demonstrated a high hybridization efficiency, staining intensity and adopt a stable duplex form with complementary nucleic acid.
In addition, mismatches at the nucleotide level between the probe on the array and the hybridized DNA can decrease the hybridization efficiency.
Simultaneously selectivity, hybridization efficiency and detection limits are maintained.
Probe hybridization efficiency was compared on two spotting surfaces: nylon membranes and epoxy-coated glass slides.
This hybridization efficiency is 3 times to 18% measured for biosensors designed with single-stranded probe.
As case study, DNA self assembled monolayer (SAM) formation and hybridization efficiency were measured.
Unfortunately, hybridization efficiency measured using such a straightaway protocol is equal to 15%, which is feeble by comparison with the 31% hybridization efficiency measured for biosensors designed with single-stranded probe.
The results show that a low probe concentration gives better hybridization efficiency and the first-hybridization conducted by a shorter-size DNA target improves the hybridization efficiency of the second target coupling onto the same probe.
Hybridization efficiency was evaluated by the attachment of fluorescent oligonucleotides to the fluorescent probe through continuous flow cytometry detection.
Hybridization efficiency in carbon fiber reinforced composites using CNTs is found to be highly dependent on the changes in the dispersion state of CNTs in epoxy.
Such modifications have showed higher hybridization efficiency but lower specificity [24].
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CEO of Professional Science Editing for Scientists @ prosciediting.com