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Repeated three-dimensional (3D) dual color fluorescence in situ hybridization combined with high-resolution cytometry was used.
The proposed strategy based on hierarchical hybridization combined with chemical bonding and interlayer engineering may hold great promise for developing advanced electrode materials for next-generation clean energy systems.
SIV gag in situ hybridization combined with immunohistochemistry for cell markers was performed as previously described [44], using a 743 bp SIVmac251 gag cDNA fragment (Genbank accession number M19499, nucleotides 1386-2129) to generate 35S-UTP-labeled riboprobes.
In the experiment of double staining (whole mount in situ hybridization combined with immunohistochemistry) the GFP expression was revealed with DAB and the embryos were mounted in glycerol and viewed with a Leica stereomicroscope.
To identify the type of cells expressing PAFR, we performed in situ hybridization combined with immunohistochemistry for the macrophages/microglia marker Iba1 (ionized calcium-binding adapter molecule-1) and the satellite cells marker GFAP (glial fibrillary acidic protein).
Catalysed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO CARD FISH) was used to determine the abundance and activity of different bacterial groups.
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The FT-RDB approach has been described by various authors for specific oligonucleotide detection and resulted to be the most efficient method for molecular hybridization, combining two advanced techniques: flow-through hybridization and simultaneous multiple detection on a macroarray membrane.
For that selection we used the following criteria: the absolute fold change should be at least 1.5 in each independent replicate; and the p-value provided by the error-model taking into account all hybridizations combined must be smaller than 10-5 to compensate for multiple testing false positives.
A method is described for HPV genotyping based on multiplex competitive hybridization (MUCH) combined with apyrase mediated allele-specific extension (AMASE).
In order to demonstrate that mRNA targeting by our probes unlikely affect their metabolism, we repeated the experiments using traditional fluorescence in situ hybridization (FISH) combined with immunostaining.
In a search for physiologically more relevant model than rodent oocytes to study this phenomenon, we have employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), to study the frequency of aneuploidy in porcine oocytes, including rare cells obtained from aged animals.
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