Sentence examples for hybridization carried from inspiring English sources

A colony blot hybridization, carried out as described with a blaIMP and a blaVIM probe (6 ), yielded a positive result with the latter probe.

The probe was chopped by carbonate hydrolysis, the embryos were fixed as above and in situ hybridization carried out according to Lehmann and Tautz (Lehmann and Tautz, 1994).

Next, equal amounts of cyanine 3- and cyanine 5-labeled cRNAs were fragmented and placed on the custom 44K salmonid microarray for hybridization carried out at 65°C for ~17 hours.

The in situ non-isotopic hybridization carried out in the present study clearly identifies α- and β-tanycytes as the main hypothalamic cells positive for GK expression; however, a reduced hybridization signal in neuronal soma (arcuate nucleus) was also detected.

Mouse and chicken embryos were rehydrated through a methanol gradient and in situ hybridization carried out for chicken β-catenin (codons 1 127) as previously described (Nieto et al., 1996).

Mouse and chicken embryos were rehydrated through a methanol gradient and in situ hybridization carried out for chicken SHH (Roelink et al., 1995), PITX2 (ChEST ChEST76c15;(Boardman et al., 2002), LEFTY1, and NODAL (kind gifts, IR Paton, the Roslin Institute, Edinburgh, UK; antisense probe- Not1, T3) FOXJ1 (Cruz et al., 2010), Lefty1/2 probe was a gift from D Norris (MRC Harwell, UK).

Bead-based hybridization carries an expectation of increased specificity over glass-based microarray [20].

Pre-hybridization and hybridization were carried out in hybridization chambers (Corning).

Pre-hybridization and hybridization were carried out at 67°C using a Denhardt 5× solution.

Pre-hybridization and hybridization were carried out under stringent conditions (21, 28).

Hybridization was carried out in hybridization chambers (Corning Life Sciences, Corning, NY) at 45°C for 16 20 h.