Sentence examples for hybridization buffer from inspiring English sources

Blots were pre-hybridized in hybridization buffer, then hybridized overnight at 42°C in hybridization buffer containing a P-labeled helena cDNA probe.

Arrays were pre-hybridized with 1× Hybridization Buffer for 10 minutes at 45°C.

The labelled probes were hybridized overnight in hybridization buffer at 65°C.

Labeled RNA were then combined, purified and hybridized in 300 µl hybridization buffer (Agilent hybridization buffer) for 16 hrs at 48°C in a dye-swap experiment.

The membrane was UV cross-linked and hybridized with PerfectHyb plus hybridization buffer (Sigma).

The probe was added to the hybridization buffer and allowed to hybridize overnight at 65°C.

Slides were prehybridized in hybridization buffer for 4 h at 65 °C and then hybridized with probes (1 μg/ml) in hybridization buffer at 65 °C overnight.

Hybridizations and washes were carried out using DIG hybridization buffer according to the manufacturer's directions (Roche).

After denaturation, libraries were diluted to 20 pM using hybridization buffer (Illumina).

After denaturation, libraries were diluted to 20 pM (hybridization buffer, Illumina).

Labeled probe (∼3 × 106 c.p.m.) in 100 μl of hybridization buffer was added to each slide (Steiner and Kitai, 2000).