Sentence examples for hybridization background from inspiring English sources
C. Microarray specificity: synthetic RNAs of hsalet- 7a, c and d were spiked into non-relevant background material (high molecular weight RNA extracted from HeLa cell line, which shows no hybridization background when hybridized to the microarray, data not shown) and hybridized to the miRdicator™ microarray.
Additionally, compared to microarray method, RNA-seq can overcome the limitation of hybridization background and different hybridization properties of different probes [48].
A sample mixed with a smaller percentage of DNA from a different genotype (strain) may increase the hybridization background signal.
Hybridization platforms are also burdened with the problems of cross hybridization, background signal, low sensitivity and limitations on the dynamic range of detection.
RMA estimates a common mean non-specific hybridization background (for an entire chip) from PM using a convolution model and then subtracts this background from PM to generate the PMrma.
We used the segmentation method to detect the presence of CDSs displaying WESs as small as 80 86% in G1-ICPB TT111 and G7-DC07-042, respiteively, in spite of high hybridization background noise (supplementary table S4, Supplementary Material online).
Thus 3× subtraction of the negative control background seems sufficient to remove the vast majority (if not all) of the methodological and non-specific cross-hybridization background.
Level of unspecific cross-hybridization, background intensity and uniformity were used to optimize hybridization and washing protocols.
These features can be exploited in order to minimize major problems in genome-wide approaches such as cross-hybridization, background and noise [ 3].
Third, hybridization probes of different genes have heterogeneous biochemical properties, which could lead to varying hybridization signal intensity levels and potential cross-hybridization background levels across genes.
This limitation comes from differences in hybridization efficiency, as well as differences in cross-hybridization background among millions of array probes and is difficult to account for.
