In situ hybridisation in combination with immunohistology was carried out as described (Herbst et al, 1997) with monoclonal antibodies specific for cytokeratins, vimentin, desmin, and smooth muscle α-actin (clones MNF116, V9, D33, and 1A4, respectively).
To prove the relevance of dominant pylogenetic groups and to localise those bacteria and archaea, fluorescent in situ hybridisation (FISH) in combination with confocal laser scanning microscopy (CLSM) was performed in samples of high-current-generating and low-current-generating Glyceria maxima (reed mannagrass) PMFC.
However, the current standard situation of dual-target hybridisations involving the combination of Cy5 and Cy3 appears to be subject to the same problem, as indicated by the single-target hybridisation with Cy5.
Studies using hybridisation techniques or a combination of different PCR assays described a higher incidence than studies using a single PCR assay [ 1, 2, 14, 16].
The chromosomal localisation of BAC_2383A24 in the allopolyploid species was determined by simultaneous in situ hybridisation using the probe combinations pSc119.2 + BAC and pAs1 + BAC.
In this paper, we investigated the potential of Ophrys hybridisation to generate novel combinations of floral traits that could induce a pollinator shift.
To do this, we utilised a complex experimental design which involves a combination of self-hybridisations and cross-hybridisations between two independently grown parasite populations for each clone being examined (for short, we call this a '2×2 CombiScan').
Test hybridisations with different combinations and ratios of mixed culture samples were set up in addition to pure culture tests in order to evaluate the performance of the microarray on community samples (see also Additional file 3, Table S3.1).
Hybridisation involves a different genomic combination in the same cells and genomes experience "genomic shock", whereas chromosome doubling doubles and restructures the genome dosage [ 11, 12].
