Plants for insect herbivory trials, leaf glucosinolate extraction, and myrosinase assays were grown in 11.5 h day/12.5 h night cycles at 22°C and 60% relative humidity (day conditions), and 16°C and 80% relative humidity (night conditions) in an environment-controlled growth room.
Open all possible doors (including trunk) - preferably on sunny, low-humidity days - for hours on end, parking your car so that it catches the most sun.
Wait for a low-humidity day.
Plants were grown in pots containing potting soil [ 69] under the environment-controlled conditions: 22 ± 1°C, 55% humidity, short days or long days, and light at 200 μM quanta/m/s.
The time to degrade to half the original modulus (t1/2) ranges from a few hours at high humidity to days at low humidity.
The combination of the heat and the humidity 365 days a year.
The microplates were then incubated (37°C, 5% CO2, 10% humidity,7 days).
For gene expression assays, rice plants were transferred to growth chambers (22 °C, 80% humidity, 16 h days) after 7 days and grown in hydroponics conditions using Hoagland solution changed twice weekly.
The synthesized MIL-101(Cr, Mg) remained excellent moisture-stability after exposed to humid air with 90% relative humidity for 30 days.
Infected plants were maintained at 16:8 h day:night cycles at 18 °C and 85% relative humidity for 21 days.
Plants were grown in a growth chamber at 25°C day, 19°C night, 50% relative humidity and 12h day length.
