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Dogs were sampled similarly (n = 36), except that all four paws were swabbed (n = 7 samples per dog taken at the same time that humans were sampled).
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Supernatants from murine BMs and splenocytes or THP-1 cells (human) were sampled for ELISA 16 h after challenge start, while those of human PBMCs and whole blood were sampled after a 24-h time period.
Human pancreas was sampled and cells were isolated as described.
No human patients or human samples were involved in the study.
Human samples were provided through the European IPF Registry and Biobank (AG).
De-identified prenatal human samples were staged, as previously described49.
Experimental procedures for the isolation of scWAT from human samples were approved by the University of Alberta Biosafety Office.
All methods, including those involving human samples, were performed in accordance with the relevant guidelines and regulations.
Human samples were obtained and handled according to the UK Home Office regulations and local guidelines in the laboratories.
Human samples were analyzed by paired t test or Wilcoxon signed-rank test.
All human samples were purchased.
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