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Since there are no established methods of non-invasively detecting neurogenesis in humans, terminal studies of nonhuman primates are the best available options to examine the clinical relevance of these findings.
In humans, terminal end bud structures are not as prominent and identifying stem cell zones has had to rely on microdissection followed by cell sorting and functional characterisation of putative cells to determine stem cell niches in ducts and lobules.
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Human terminal exon sequences matched rhesus contigs with a 94.4% mean identity.
Mean alignment size was approximately 1,100 bp covering greater than 90% of each human terminal exon.
In our description of our method below, we use the human terminal lineage as an example.
In both cases the main focus was the human terminal lineage after its divergence from chimpanzee.
We will call the human lineage after the divergence of chimpanzee the human terminal lineage, and the human lineage after the divergence of macaque (e.g the human terminal lineage plus internal branch 1) the long human lineage.
These elements, which are generally very conserved among vertebrates, are changing extremely rapidly on the human terminal lineage.
The equivalent structures for human terminal ductal lobular units and lobuloalveolar units in rodents are terminal end buds (TEB) and alveolar buds, respectively [ 10].
In addition, we aligned human terminal exons against the MacaM assembly to extend rhesus terminal exon annotations through the 3′ UTR to the end of the exon.
Using human terminal ileal biopsy tissues they show that intestinal permeability to solutes, but not macromolecules, was significantly increased in the intestines of elderly humans.
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