I think there's something human in sheet music, something that doesn't depend on technology to facilitate it — it's a way of opening music up to what someone else is able to bring to it.
Total RNA extracted from human epidermal sheet, whole skin, and cultured cells using RNeasy Micro Kit (Qiagen, Valencia, CA, USA) were used for the single-stranded cDNA synthesis by High-Capacity cDNA Reverse Transcription Kit (Life Technologies).
In our preliminary study, we could successfully vitrify cell sheets with more fragile characteristics including human chondrocyte sheets.
Cultured human periosteal sheets more effectively function as an osteogenic grafting material at implantation sites than do dispersed periosteal cells.
In order to alleviate their extensive contraction, human fibroblast sheets were cultured in combination with three-dimensional matrices (knitted poly(lactic-co-glycolic acid) (PLGA) mesh and collagen hyaluronic acid (CHA) sponge) to form contiguous dermal constructs for tissue engineering a bilayered skin equivalent.
We therefore infected human epidermal sheets with different concentrations of HSV-1-GFP (MOI of 0.3, 3 and 30).
The purpose of this study was to further investigate the properties of human chondrocyte sheets using scanning electron microscopic evaluation and gene expression and immunohistochemical analyses.
Human fibroblast sheet-derived vascular grafts were also tested in nude rats (long-term study) and primates (for up to 8 weeks), showing physiological mechanical strength and positive scaffold remodelling with activation of resident cells [ 111].
In this study we utilized a human MSC cell sheet and seeded HUVECs onto the cell sheet, which provides a vascularization microenvironment and also has stem cells sources for tissue differentiation.
Human myofiber and sheet architecture is modeled using eigenvectors computed from diffusion tensor MR images from an isolated, fixed human organ-donor heart and transformed to the patient-specific geometric model using large deformation diffeomorphic mapping.
