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In the museum's human section, dozens of brains were presented in various cross-sections.
Formalin-fixed sections (human section) were, then, treated with target retrieval solution (DAKO, Carpinteria, CA) for 10 min in steamer; no target retrieval treatment for zinc-based Beckstead's solution-fixed mouse tissues.
We compared our results with data provided in the human section of dbEST.
Small images from the human section were acquired without pixel binning.
Searches were performed against UniProtKB/Swiss-Prot (vers. 56.1) [ 29] human section, including all the isoforms.
It undertook to do so by signing the Barcelona Declaration, because the Euro-Mediterranean partnership was made up of three sections, a political and security section, an economic and financial section and a social, cultural and human section.
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Slides were developed using DAB (human sections, Sigma) or AEC (murine sections, Sigma).
The slides were counterstained with methyl green (human sections, Sigma) or hematoxylin (murine sections).
Sections were stained for TLR2 (anti-mouse/human TLR2, T2.5, eBioscience), incubated with powervision block (human sections, Immunologic), powervision poly-HRP-GAM/R/R IgG (human sections, Immunologic) or goat anti mouse IgG1 HRP (murine sections, Southern Biotech).
The number of cells positive for TUNEL, DAPI, and CHOP (human sections only) were counted from four fields obtained from each section.
Serial sections were routinely processed as previously described for the human sections.
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