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Ian Wilmot, Scotland's cloner of Dolly, is an informed opponent of human replication; he knows how many of his attempts to clone sheep failed, and believes similar attempts with humans would be horrifying.
The JCN algorithm achieved an optimal correlation with human judgments of 0.87, compared to a human replication correlation of 0.88 and correlations of 0.82 and 0.60 for the node-based and edge-based approaches respectively [18].
The detection of human replication N-domains is based on the mammalian replicon model [ 58, 59].
The coordinates of the 678 human replication N-domains were obtained directly from the authors [ 46].
Gene Gnf1 is a 1-to-1 ortholog of the human replication factor C subunit 1 (RFC-1) gene, which encodes a major methotrexate transporter.
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In another approach, a human replication-incompetent, adenoviral vector based influenza vaccine could be developed, in which genes of anti-inflammatory cytokines are coexpressed, which will inhibit overproduction of proinflammatory cytokines.
In humans, replication protein A is the best-understood member of this family and is used in processes where the double helix is separated, including DNA replication, recombination and DNA repair.
These findings support the role of DNA ligase III as the replicative DNA ligase in human mtDNA replication and suggest that DNA ligase III functions in the maturation process of Okazaki fragments.
The mechanisms of human mtDNA replication are far from resolved, and several origins of replication have been proposed.
In human the replication dependent variants are represented by H3.1 and the replication-independent variants best characterized are H3.3 and centromeric H3 variants (CenH3) [ 4].
Our laboratory has been studying the roles of modular multidomain proteins involved in human DNA replication using SAXS and NMR.
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