Exact(2)
Many have tried, the most zealous inventing statistics with names like Defensive Linear Weights, Zone Rating and the like, but fielding skill has proven too elusive for human quantification.
DNA concentrations were determined by Real-Time PCR on an ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA) with the Quantifiler® Human Quantification Kit (Applied Biosystems, Foster City, CA).
Similar(58)
This paper proposes a modified CREAM to facilitate human reliability quantification in marine engineering by incorporating fuzzy evidential reasoning and Bayesian inference logic.
Human DNA quantification has become an essential means to evaluate the preservation of ancient samples and to ensure the authentication of the results in ancient DNA research.
The method is based on human performance quantification and is supported by a tool that enables scenario-based evaluation of prospective organisational processes through simulation.
Human mtDNA quantification was accomplished by monitoring the real-time progress of the PCR-amplification of two different fragment sizes (113 and 287 bp) within the hypervariable region I (HV1) of the mtDNA control region, using two fluorogenic probes to specifically determine the mtDNA copy of each fragment size category.
DNA extracts were quantitated using the Quantifiler™ Human DNA Quantification kit (Applied Biosystems, Foster City, CA) according to manufacturer's protocols.
DNA concentration was determined by real-time PCR using the Quantifiler™ Human DNA Quantification Kit (Applied Biosystems).
A limitation to the human DNA quantification method using a qPCR assay is that it may be sensitive to unknown PCR inhibitors present in greater concentration in the cloudy samples.
DNA extracts were quantified by real time PCR, using an ABI Prism 5700 Sequence Detection System (Applied Biosystems, USA) and the Quantifiler® Human DNA Quantification Kit (Applied Biosystems, USA) according to the manufacturer's protocol.
In the present study, we developed a novel reverse-transcription real-time PCR assay for human miR-499 quantification.
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