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In 1987, Bedford and van Helden analysed DNA 5′-methylcytosine content in human prostate samples and reported a correlation between global hypomethylation and development of benign prostatic hyperplasia (BPH) and metastatic tumours (Bedford and van Helden 1987).
Seven human prostate samples were obtained by transurethral resection from the suspected benign hyperplasia patients.
Finally, we were interested in assessing the mRNA levels of α1A-AR and cav-1 in a series of cancerous and hyperplastic human prostate samples.
We are particularly grateful to Pr. Morad Roudbaraki for RNA extraction from human prostate samples and information obtained from the hospital's anatomopathology department.
Two publically available datasets were used to examine AGR2 gene expression in human prostate samples [ 31, 32].
Validation was performed first on seven human prostate samples that underwent whole genome sequencing (WGS) [ 20] and was then extended to a larger set of 90 samples that underwent whole exome sequencing (WES) [ 21].
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The different SHBG sequences were amplified by PCR from cDNA of a human prostate sample using the primer pairs described in the supplementary table (where the inserted restriction sites are underlined).
To test the capability of eRNA for mRNA data analysis, ten mRNA samples were extracted from normal human prostate tissue samples and sequenced.
Real-time RT-PCR revealed that the expression levels of let-7a were decreased by ∼43% in resected human prostate cancer samples compared to the adjacent non-cancerous samples.
We performed single-end RNA-Seq on three human prostate adenocarcinoma samples and their corresponding normal tissues, as well as brain and universal reference samples.
The RGS17 and ASCL2 mRNA levels were assessed by RNA-seq in TCGA, Illumina Expression BeadChip-based transcriptional profiling in Camcap and Stockholm cohorts of human prostate tissue samples.
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