Resected lung from patients with COPD undergoing lung cancer surgery was profiled using miRNA (Agilent Human miRNA profiler G4470 V1.01) and mRNA (OperonV2.0) microarrays.
Culture supernatants were analyzed by using the proteome profiler human angiogenesis array (R&D Systems, Minneapolis, MN), according to the manufacturer's instructions.
The proteome profiler Human Cytokine Array Panel A Array Kit (R&D Systems) was used according to the manufacturer's instructions.
Human apoptosis proteome profiler assays were performed to investigate the mechanism of cell death.
RT² Profiler Human Preeclampsia PCR Array (PAHS-163Z, Qiagen, Valencia, CA) was used for gene expression profiling.
The analysis for specific proteins was performed using proteome profiler human arrays (Catalog No. ARY007, R&D Systems Inc., Minneapolis, MN, USA), according to the manufacturer's instructions.
The Proteome Profiler Human Phospho-Kinase Array Kit (Cat. No: ARY003B) and Human Angiogenesis Array Kit (Cat. No: ARY007) were obtained from R&D Systems (Minneapolis, MN, USA).
For the assessment of MAP kinase activation we employed the proteome profiler human phospho-MAPK array kit (R&D Systems, Minneapolis, MN, USA, ARY001B).
Human apoptosis proteome profiler antibody arrays (R&D Systems, Abingdon, UK) were performed in accordance with the manufacturer's instructions using 200 μg total protein.
The human mTOR RT profiler PCR pathway array (SABioscience Qiagen, Cat.no.330231 PAHS-098ZD) was used to validate mTOR regulation identified through RNA seq.
