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Nicke, B. et al. Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells.
To obtain proof-of-concept that locally produced anti-inflammatory steroids suppress ovulation-associated extracellular matrix proteases in human ovarian surface epithelial (OSE) cells.
In this novel paradigm of cancer therapy, the statistically significant difference in killing ovarian cancer cells versus healthy human ovarian surface epithelial and artery endothelial cells is of paramount importance for pursuing clinical trials.
An immortalized human ovarian surface epithelium cell line was used as non-transformed control.
Additionally, GATA4 expression was variable in primary cultures of human ovarian surface epithelial cells.
We found that p110α was highly detectable in the early malignant transformed human ovarian surface epithelium (Figure 2A and B).
The panel of human "immortalized" ovarian (HIO) epithelial cell lines [17], two preparations of primary human ovarian surface epithelial cells [36], and ovarian cancer cell lines were analyzed.
One of the unique features of our study is the use of primary cultures of normal human ovarian surface epithelium as the normal comparison group.
Normal human ovarian surface epithelial lines (HOSE) and CaOV3 and ES-2 cancer cell lines were maintained in DMEM containing 10% FBS and 1% penicillin/streptomycin cocktail.
Isolation of human ovarian surface epithelial cells is based on the relatively loose attachment of surface ovarian epithelial cells to underlying structures [3], [37].
We isolated primary human ovarian surface epithelial (HOSE) cells and human breast epithelial (HBE) cells from normal tissues obtained from non-disease related surgeries.
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