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Arguing against this is the fact that we were unable to detect any evidence for capillary congestion by cells using immunostaining for human nuclei, where we saw human nuclei-positive cells evenly scattered in regions containing marrow cells and not inside blood vessels.
An embryo whose cells have human nuclei and rabbit mitochondria is not a monster.
(3) The gene-richest chromosomal regions display a much more spread-out conformation compared to the gene-poorest regions in human nuclei.
Muscle sections were air-dried and immunostained against human dystrophin, human nuclei and human PECAM [12].
The merged image of human insulin and human nuclei immunostaining was shown in Figure 7B-d.
The number of human nuclei was determined in every eleventh section throughout the striatum.
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The cells were then fixed in 10% formalin and stained with anti-human nuclei and anti-MHC antibodies.
The immunohistochemistry identification of human cells was performed by using a primary mouse anti-human nuclei monoclonal antibody (Chemicon), specific for all human cell types.
And they were incubated overnight at 4°C with the following primary antibodies and dilutions: mouse anti-human nuclei monoclonal antibody (Chemicon, 1∶100); guinea pig anti-human insulin antibody (Linco Research, 1∶100).
To analyze the survival of transplanted hUCB-MSCs, we double stained using an antibody for human-specific anti-human nuclei (green) and human β2-microglobulin (red) at 10, 20, and 40 days.
Slices were incubated with the following antibodies: mouse anti-MBP (Sternberger Monoclonals Inc, Lutherville, MD), chicken antineurofilament medium chain (NFM) (EnCor Biotech, Alachua, FL) and mouse anti-human nuclei (Millipore) diluted in blocking solution for 48 h at 4°C.
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