TRP-1 expression was verified by indirect immunostaining of Ad5.TRP-1-infected human HEK293T cells (multiplicity of infection (MOI) = 1) using supernatant of the TRP-1-specific hybridoma TA99, kindly provided by Alan N. Houghton Sloan Kettering-Institutee, New York, NY), together with a goat anti-mouse-Cy3 conjugate (BD Pharmingen, Heidelberg, Germany).
Implications and future directions The gpA33 ΔN-Bcat mice provide a model that better mimics some aspects of sporadic colon cancer induction in humans, including tumor multiplicity and latency and site-specific (i.e. colon) tumor occurrence; in addition, these mice show upregulation of markers of colorectal cancer progression in humans.
There should be a way to take into account the multiplicity of human cultures and to describe human weaknesses without the application of such highly charged systems, without simply invoking their now-familiar rosters of victims and heroes.
I think it can express the multiplicity of human beings really well".
And though the catalog of emoji has expanded in response to user demand, it still struggles to keep up with the multiplicity of human experiences.
Pegg concluded by urging that Sulu's sexuality shows the multiplicity of human experience across the space-time continuum, showing that "we are all LGBT somewhere".
It is things, the furniture of the universe, that are particular and variable; the multiplicity of human observers melts into an undifferentiable mode of response.
Fine recent novels like Jennifer Egan's "Visit from the Goon Squad" and Tom Rachman's "Imperfectionists" also take wide-angle views, using large casts of characters to explore a multiplicity of human experience.
And because it is feminist, it must promote the elimination of gender inequality and take into account the multiplicity of human bodies, desires, and differences that are mapped onto the site of religion.
The original unity of king and priest, which derives from the divine unity, permits human beings, after the multiplicity of sects introduced by the devil and fomented by ambition and ignorance, to return to a single priestly law, under which the entire human race can come together, transcending divisions and conflicts.
