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The present study determined the response of both primary murine bone marrow derived macrophages (BMDM) and a transformed human mononuclear cell line (THP-1 cells) to degradation products of two different, commonly used ECM bioscaffolds; urinary bladder matrix (UBM-ECM) and small intestinal submucosa (SIS-ECM).
human mononuclear cell line.
Activation of T lymphocytes in human mononuclear cell cultures was monitored by measuring release of IFNγ in response to SEB pre-incubated with each antibody.
The expression of MMPs in human mononuclear cell subsets has been systematically analyzed in a previous study by Bar-Or et al [24] showing that MMP-9 mRNA was particularly abundant in monocytes compared with B and T cells.
Human mononuclear cell line, THP-1 (ATCC TIB-202), was used in our experiments.
To assess the role of survivin in the inflammatory process, the human mononuclear cell line THP-1 was transfected with oligonucleotides targeting different regions of survivin mRNA.
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Phagocytosis of killed endospores by glass adherent peripheral human mononuclear cells was studied.
In human mononuclear cells, (BCG + HBV) enhanced mRNA expression of several genes including CSF2, which contributed to clustering of genes by vaccine treatment via principle component analysis.
Isolated human mononuclear cells were cultured at 4 × 105/mL in 24 well plates over a period of two days after which they were incubated with microbes (4 × 106 CFU/ml) for 4 h at 37 °C and 5 CO2.
Moreover, serum lipid antioxidant capacity and anti-inflammatory potential in human mononuclear cells were tested as more biologically relevant assays than the chemical radical scavenging assays.
The objective of this study was to evaluate the kinetic parameters at equilibrium of peripheral benzodiazepine receptors (PBR) in human mononuclear cells from patients affected by osteoarthritis (OA), rheumatoid arthritis (RA) and psoriasic arthritis (PA).
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