Briefly, myeloma cells were adjusted to a concentration of 1 × 10 per ml cells using human methylcellulose-based medium (R&D Systems Inc).
CFUs were performed using methylcellulose base media as described recently.
After transfection, about 1000 cells were subjected to the methylcellulose colony formation assays in triplicate using human methylcellulose media (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's instructions.
We also cultured P1A Tg BM cells and control cells in methylcellulose based medium to quantify potential progenitors in vitro.
A pre-made methylcellulose based colony assay medium (Stem Cell Technologies, Canada) was used as described by the manufacturer.
To quantify lineage-restricted and multipotential progenitors, MethoCult methylcellulose-based mediums were used (StemCell Technologies, Vancouver, BC, Canada).
The study of Economidou and coworkers [ 90] set out to evaluate the effects of administration of two (unidentified) commercial membranes: a thicker membrane composed of macromolecular polysaccharides and a thinner HA-hydroacid methylcellulose-based membrane.
Fifty microliters of blood from mice was lysed and plated in human complete methylcellulose medium and scored at day 14 as previously described.
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