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Both PAD2 and PAD3 proteins could be detected by Western blot in the developing human brains and spinal cords at the stages tested, but their levels of expression were more difficult to quantify given the limited amount of human material available (Fig. 1C).
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Additionally, when human material is available, genomics and proteomics studies are inevitably complicated by sources of variation from maternal, fetal, and clinical origin.
Due to the ILM removal, human material became available and was used to study sequestered factors, which could be involved in the pathogenesis of early diabetic retinopathy [ 21].
5-HT1F immunoreactivity was not observed in the human material (using the available antibodies).
This study involved no patients or human material, was lawfully available and in compliance with the Helsinki Declaration.
We converted acrolein concentrations used by Costa et al. (1986) into human equivalent concentrations (HEC) by converting from intermittent exposure to continuous exposure, from shorter to longer duration of exposure, and from rat physiology to human physiology (Supplemental Material available online at http://www.ehponline.org/docs/2006/9467/suppl.pdf).org/docs/2006/9467/suppl.pdf
Approximately 24% of the sequenced clones were closely related to potential human, plant, and animal pathogens (Supplemental Material available online at http://www.ehponline.org/members/2007/10684/suppl.pdf).org/members/2007/10684/suppl.pdf
Human fetal gonads were harvested from material available after legally induced abortions in the first trimester of pregnancy (i.e., from the 7th until the 12th week postconception) in the Department of Obstetrics and Gynecology at the Hôpital Antoine Béclère, as previously described (Lambrot et al. 2006a, 2006b).
The age/hormonal status of the donor for cell/tissue may not be available depending on the source of the material (that is, abattoir, human material for which medical information/records are not available, some immortalized cell lines).
Many amino acids are needed to be optimized for expression in human cells (more than 79%) (see Supplementary Material available online at http://dx.doi.org/10.1155/2013/384091).org/10.1155/2013/384091
Formalin-fixed paraffin-embedded (FFPE) tissues represent the largest source of archival biological material available for genomic studies of human cancer.
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