Experiments with human fibroblasts provided evidences that tumour DNA may be horizontally transferred by the uptake of apoptotic bodies (Holmgren et al, 1999).
This composition indicates the observed free radical scavenging activity and the effects of pollen on human fibroblasts and provides new insights towards enhancement of cellular antioxidant mechanisms by natural products as bee-pollen.
Recent studies in human fibroblasts have provided a new general paradigm of tumor suppression according to which oncogenic signaling produces DNA damage and this, in turn, results in ATM/p53-dependent cellular senescence.
The ability to generate male germ cells from human iPS cells derived from fibroblasts provides an excellent paradigm for elucidating the mechanism of gametogenesis as well as for developing new approaches for treating male infertility.
Investigations using human A549 lung cancer cells, FaDu hHNSCC cells, human HL60 leukemia cells or mouse fibroblasts provided evidence that cells either overexpressing a constitutively active kinase form of ILK or expressing a wildtype form of ILK are significantly more sensitive to irradiation than their normal or ILK knockout counterparts, respectively.
In human skin, stromal cells such as dermal fibroblasts provide a crucial microenvironment for epidermal keratinocyte function (Blanpain and Fuchs, 2009).
GM701 (immortalized human fibroblasts) cells kindly provided by Dr. M. King (Thomas Jefferson University) were cultured in Dulbecco's Minimum Essential Medium (DMEM; Gibco Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and antibiotics.
A similar approach used a combination of human vascular SMC-derived sheets and human fibroblasts sheets to provide the adventitia.
The results of their animal study, which were later confirmed with normal human fibroblasts [ 104], provided unequivocal evidence that short (presumably near-dysfunctional) telomeres increase sensitivity to radiation.
Primary cultures of human fibroblasts were kindly provided by Dr Y Kuroyanagi (R&D Center for Artificial Skin, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan) and similarly cultured in Dulbecco's modified Eagle's medium (Gibco).
