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Stage five is less about keeping your small human fed, clean, and alive and more about keeping them entertained.
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Although sand flies recently fed on chickens contain nearly double their normal DNA content compared to human-fed sand flies due to the nucleated avian erythrocytes (unpublished data), chicken blood meals could not be identified after 48 h (Figs. 1B and 2B).
δN was the most informative variable and could distinguish unfed, chicken-fed and human-fed mosquitoes.
Time-course analysis on chicken and human-fed sand flies in the laboratory showed that host DNA could be detected for up to 48 h after the blood meal (Fig. 1).
The time-course experiments showed that host DNA was detectable in chicken and human-fed control sand flies up to 48 h after the blood meal (Figs. 1 and 2).
It is, therefore, possible that persistent differences observed between formula-fed and human milk-fed individuals are the result of epigenetic alterations induced by subtle nutritional differences between human milk and infant formula' (Waterland et al, 2006, p. 712).
(3) This experiment with 13C-α-limit dextrins needs to be repeated in human breast fed infants.
Linear regression model with and without baseline serum lutein indicated baseline serum concentrations did not have a significant effect on final serum concentrations in either the formula (P < 0.001) or human milk fed groups (P = 0.001).
A total of 40 infants were enrolled, including 14 human milk-fed and 26 formula-fed infants (Table 1).
Forty seven human blood-fed, 49 chicken blood-fed and 58 wild-caught blood-engorged sand flies were homogenised individually in 50 μL of 0.15 M saline using a cordless motorized pestle and the whole volume spotted onto a Whatman® FTA card.
Briefly, 20 human blood-fed and 30 chicken blood-fed sand flies were homogenised individually in 1.5 mL microcentrifuge tubes with 244.5 μL sodium phosphate buffer and 30.5 μL MT buffer (both provided with the kit) using a cordless pestle pellet motor on ice.
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