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While preserving this essential role, higher eukaryotic aaRSs, such as human cytoplasmic aaRSs, have developed other functions during evolution, including angiogenesis, inflammation, development, tumorigenesis, etc.
The human cytoplasmic protein tyrosine phosphatase (HCPTP) has been identified as a potential target for inhibition in order to downregulate metastatic transformation in several human epithelial cancers such as breast, prostate and colon cancer.
The homologous mammalian ribosomal proteins carry Gln-87 (humitochondrialribosomessores) or Gly-87 (human cytoplasmic ribosomes) in part accounting specificity of drugs for prokaryotic ribosome [7].
To visualize human myelinated host axons, we performed double immunofluorescence and confocal analysis for the human cytoplasmic marker SC121 (red) and CASPR (green) on parasagittal spinal cord sections 16 weeks following transplantation (Fig. 6).
Because SC121-positive cells in the white matter predominantly differentiated into oligodendrocytes, co-localization of the human cytoplasmic marker SC121 with the paranodal protein CASPR within the white matter of the spinal cord is suggestive of host mouse axons remyelinated by hCNS-SCns that have differentiated into oligodendrocytes.
Their numbering is as described for the human cytoplasmic ribosome (Anger et al., 2013 ).
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As observed in humans, cytoplasmic inclusions in Pank2 −/− mice were negative for α-synuclein, confirming that PKAN neuropathological findings are different from other forms of neurodegeneration with brain iron accumulation.
The following datasets were generated: Jia X, Xiong Y, 2014, Crystal structure of the human BST2 cytoplasmic domain and the HIV-1 Vpu cytoplasmic domain bound to the clathrin adaptor protein complex 1 (AP1) core, http://www.rcsb.org/pdb/explore/explore.do?structureId=4P6Z, Publicly available at the RCSB Protein Data Bank (http://www.rcsb.org/ ).
Using specific constructs and peptides of human EGF cytoplasmic domain, we demonstrate that EGFcyt facilitates lysosomal and proteasomal protein degradation, and this coincided with growth inhibition of human thyroid and glioma carcinoma cells.
Tani et al. (2001) have reported that substitution of a non-polar amino acid at position -3 in the human α6A cytoplasmic domain reduces the interactions with GIPC by 60%.
The polyclonal antiserum to recombinant human megalin cytoplasmic domain (anti-MegT) was previously described [7].The secondary antibodies for immunostaining and PAP complex were purchased from ICN Biomedicals, Inc. (Aurora, OH).
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