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GST fusion proteins of RB (human, containing amino acids 395 through 928), E2F1 (human, full-length), E2F3 (human, full-length), DP1 (human, full-length) and E7 (human papilloma virus-16, full-length) were expressed in bacteria and purified on Glutathione Sepharose (Pharmacia) by standard methods [8], [16], [17].
We obtained FASTA files for mouse (containing 585 sequences) and human (containing 868 sequences).
Briefly, using bioGPS website (http://biogps.org/ goto=welcome), a Gene Atlas averaged dataset of expression values based on HGU133 array for human containing 84 tissues (Su et al., 2004) was used.
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Lymphoid tissues in mouse contain pDC, CD8α+ cDC and CD4+ cDC, while lymphoid tissues in human contain pDC, CD141+ DC and CD1c+ DC.
Non-lymphoid tissues in mouse contain CD103+ DC and CD11b+ DC, while non-lymphoid tissues in human contain CD141+ DC, CD1c+ DC and CD14+ DC.
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84% of the contigs that found a match in human contained an ORF longer than 300 bp.
Even human contains a pseudogene for CXCL15 (our unpublished result).
Further, in 40% of all primate clusters, those from human contain more C2H2-ZNF genes than those from chimpanzee.
Next, the assay was evaluated for its specificity by testing sera from humans containing antibodies against the heterologous flaviviruses, TBEV and DENV.
C. trachomatis is primarily a pathogen of humans, containing the trachoma biovariant (biovar), and the lymphogranuloma biovar (LGV).
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