However, it must be outlined that although effective in detecting pathogens, these sampling plans are difficult to be applied since the huge number of samples that needs to be tested.
The convergence needed a huge number of samples.
Currently, automated high-throughput capillary electrophoresis instruments are available and are helpful for handling a huge number of samples.
By using pre-processing stages described in Section "Pre-processing systems", and huge number of samples, fairly average results have been shown.
In addition, to validate the obtained results, a huge number of samples are necessary, which generally are not always available through in situ measurements because of they require considerable costs and time.
This is a large dataset with a large number of classes (1,000 classes) and a huge number of samples (more than 1 million samples), and the density of dataset is about 6%% (the proportion of non-zero elements of dataset in percent).
It was concluded that optimization of k can be a time-demanding procedure especially in the case of a huge number of samples, but it was also observed that this approach is quite insensitive to the smoothing parameter k, except for very small k values which led to the results influenced by local noise.
In order to sequence a huge number of samples in a single sequencing run, a stable and reliable method for identifying the generated reads is required.
We are well aware that Genome-Wide Association studies (GWAs) represent a powerful tool to explore variants associated to complex traits and to evaluate possible gene-gene interactions [14], but the huge number of samples needed to reach the genome-wide threshold of significance cannot be realistically applied to an endophenotype like salt-sensitivity, which requires great care in measurement.
The sequencing process manifold to permit concurrent sequencing each reaction and to analyse the huge number of samples.
