Exact(1)
The fluctuating nature of blood from different individuals, huge dynamic protein concentration ranges (up to 10), and the protein detection limits of most MS platforms, have made the plasma proteome elusive to define.
Similar(7)
The huge dynamic range of protein concentrations, rapid turnover and the fact that many proteins are not excreted into the circulation probably contribute to this.
Today, advances in imaging technology, such as cryo-electron microscopy and free-electron lasers, allow scientists to freeze protein samples to image huge, dynamic molecular structures or to collect the information from instantaneous "diffract and destroy" of tiny droplet samples using electron blasts.
However, the huge dynamic range in the amounts of the individual protein components in plasma (>10 orders of magnitude in difference) hampers the discovery as well as routine determination of novel biomarkers, since typical plasma proteomics is biased towards the detection of primarily high-abundance proteins.
The proteomic data set is, however, logistically limited due to huge dynamic ranges, with a natural bias against low abundance and transmembrane proteins (Borg et al. 2011).
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Zhang, L., Borthakur, S. & Buck, M. Dissociation of a dynamic protein complex studied by all-atom molecular simulations.
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